Fluorescence Microscopy Platform
The Time-resolved confocal fluorescence microscope is adapted for different types of measurements/modes of data analysis: FLIM, FCS, FRET, measurement of fluorescence lifetimes in liquid or solid samples, unimolecular spectroscopy, nonlinear optics measurements, monitoring of fluorescence signal fluctuations. Possible applications: FLIM on biological structures (cells, tissue) or materials with luminescent properties; characterization of the interaction of some exo- or endogenous chromophores with (plasmonic) nanoparticles applied in therapy, diagnosis and imaging; Cell biology; Non-linear optics; Two-photon imaging. The confocal microscopy system (Re-scan Confocal Microscopy - RCM) is based on the "double scan" method (a scan of the laser beam simultaneously with a scan of the beam emitted by the sample). This innovative method leads to obtaining a much better lateral resolution than in standard confocal microscopy. This peculiarity of scanning and respectively high resolution gives to our microscopy system a high degree of uniqueness both nationally and internationally; 2 re-scanning RCM units: for the NIR domain, with lateral resolution of 240 nm (RCM-NIR) and 140 nm respectively for the visible domain (RCM-VIS); the RCM-NIR unit is equipped with two laser diodes with emission lines at 640 and 785 nm, respectively.
(BBU) Babeș-Bolyai University, Monica Focsan
FLIM
FRET
FCS
Two-photon excitation
Nonlinear optics
Unimolecular detection sensitivity
High-resolution confocal fluorescence (bio)imaging
3D fluorescence imaging
NIR confocal imaging
Immunofluorescence
Intracellular uptake studies
Babeș-Bolyai University